Excellent and state-of-the-art lab facilities allow scientists at Mech-Sense to perform efficient and high quality research from developing and validating new models and translating them into pharmacological interventions and patients.
Facilities at Department of Radiology
A 3T system and several 1.5T MRI systems allows for:
Advanced brain imaging:
- 3D brain structure for volume and cortical thickness analysis
- DTI for assessment of white matter tracts and structural connectivity
- Resting state fMRI for functional connectivity analysis
- Task related fMRI for assessment of brain activation
- Spectroscopy for assessment of concentration of brain metabolites
Imaging of pancreas:
- Structural imaging:
- Gland volumetry
- DWI for fibrosis assessment
- Dixon for fat assessment
- Ductal changes
- Functional imaging:
- Secretin stimulation for assessment of exocrine pancreatic secretion
- Colonic volume
- Colonic content
- Gastric emptying
- Small bowel motility
- Small bowel water content
Facilities at Department of Gastroenterology and Hepatology
Our fully equipped laboratory allows for the application of a range of methods, some of which are listed here:
Resting and evoked EEG can be measured at the cervical as well as cortical level using standard EEG equipment.
Application of different probes for either esophageal or rectal stimulation that allows following visceral stimuli:
- Mechanical stimuli with water or air infusion into a small balloon mounted on a probe.
- Thermal stimulation (by circulating warm or cold water in the balloon (1°C, 68°C).
- Electrical stimulation (stimulation of the mucosa with weak electrical impulses (below 80 mA) through electrodes on the probe.
- Chemical stimulation by perfusion of hydrochloride acid with the same pH concentration of stomach fluid or a capsaicin solution.
QST (quantitative sensory testing):
- Thermal stimulation: Thermal detection and pain thresholds assessed using a computerized contact heat-evoked potential stimulator.
- Mechanical stimulation: Pressure pain threshold assessed using an electronic pressure algometer.
- Sensory testing: Allodynia and hyperalgesia assessed with validated methods such as Pinprick and/or soft brush, or Von Frey monofilaments.
- Cold pressor test in combination with test pain: CPM (conditioned pain modulation) is assessed by scoring pressure pain before and after submerging the subject’s hand into 2ºC water for two minutes.
- Electrical stimulation: Nociceptive withdrawal reflex. Electrical stimulation of the median nerve at the wrist.
- Cardiovascular autonomic reflex test using the handheld VagusTM device
- Heart Rate Variability from 24 hour and 5 day sternum based electrocardiogram
- Cardiac vagal tone from five-minute electrocardigraphic readings
- Sudomotor function
Whole gut motility assessments:
Capsule-based assessment methods that include tracking of small non-digestible capsules through the gastrointestinal tracts. The measurements allow for assessment of segmental gastrointestinal transit times.
- SmartPill motility capsule investigation that measures pressure, pH, and temperature. Motility indices can be derived from individual segments.
- 3D-Transit motility capsule investigation that measures rotation and 3D position of the capsule. The capsule can be live-tracked using a bluetooth connection and a fast (5-10 Hz) sampling frequency allows for quantification of capsule movements including length, direction and velocity.
Modulation of the pain system: Breathing techniques, physical activities and distraction can be used to modulate the pain system. Blood samples for PK/PD (pharmacokinetic/pharmacodynamic modeling): Samples are taken and stored at -80°C for further biochemical analysis elsewhere.
Pupil eye diameter: Measured with pupilometer. Pupil eye diameter is e.g. a sensitive assessment of the presence of opioids in the CNS and can be correlated to changes in CNS